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中级

丁丽华

讲师、硕士生导师

研究方向:生物样品分析新方法新技术

邮箱:zzulihuading@zzu.edu.cn


一、教育/工作经历

工作经历:

[1] 2022-至今,郑州大学公共卫生学院,讲师

教育经历:

[1] 2018-2022,郑州大学,劳动卫生与环境卫生学,博士

[2] 2015-2018,郑州大学,营养与食品卫生学,硕士

[3] 2010-2015,郑州大学,预防医学,本科

二、主持科研项目

[1] 国家自然科学基金青年项目(编号:82304194;年限:2024-2026):适配体介导膜融合联合CRISPR-CHA的外泌体膜蛋白和miRNA高灵敏同步检测传感器构建及应用

[2] 中国博士后科学基金特别资助(站前)(编号:2023TQ0304;年限:2023-2025外泌体中多种肺癌相关标志物同步检测体系建立及临床应用

[3] 中国博士后科学基金面上项目(编号:2023M743196;年限:2024-2025:基于CRISPR/Cas9和CHA的外泌体多种蛋白高灵敏检测体系构建及其在非小细胞肺癌智能监测中的应用

[4] 中原青年博士后创新人才项目年限:2024-2025): 外泌体多维生物标志同步检测及其联合CT影像的肺癌精准诊断

[5] 郑州大学求是青年教师科研启动基金年限:2023-2025): 外泌体高灵敏检测体系构建及应用


三、代表性成果

l 论文

[1] Ding LH, Liu X, Zhang ZZ, Liu LE, He ST, Wu Y, Effah YC, Yang RY, Zhang AA, Chen WS, Yarmamat M, Qu LB*, Yang XN*, Wu YJ*. Magnetic-nanowaxberry-based microfluidic ExoSIC for affinity and continuous separation of circulating exosomes towards cancer diagnosis. Lab on a Chip 2023, 23: 1694-1702.

[2] Ding LH, Wu Y, Liu LE, He LL, Yu SC, Effah YC, Liu X, Qu LB*, Wu YJ*. Universal DNAzyme walkers-triggered CRISPR-Cas12a/Cas13a bioassay for the synchronous detection of two exosomal proteins and its application in intelligent diagnosis of cancer. Biosensors and Bioelectronics 2023, 219:114827.

[3] He ST#,Ding LH#, Yuan HJ, Zhao GF*, Yang XN*, Wu YJ*. A review of sensors for classification and subtype discrimination of cancer: Insights into circulating tumor cells and tumor-derived extracellular vesicles. Analytica Chimica Acta 2023, 1244: 340703.

[4] Ding LH, Yang XN, Gao ZB, Effah YC, Zhang XJ, Wu YJ* Qu LB*. A holistic review of the state-of-the-art microfluidis for exosome separation: An overview of the current status, existing obstacles, and future outlook. Small 2021, 17(29): 2007174.

[5] Ding LH, Liu LE, He LL, Effah YC, Yang RY, Ouyang DX, Jian NG, Liu X, Wu YJ*, Qu LB*. Magnetic-nanowaxberry-based simultaneous detection of exosome and exosomal proteins for the intelligent diagnosis of cancer. Analytical Chemistry 2021, 93(45):15200-15208.

[6] Ding LH, Wu YJ, Duan YJ, Yu SC, Yu F, Wang J, Tian YM, Gao ZB, Wan ZZ, He LL*. A novel cell-assisted enhanced chemiluminescence strategy for rapid and label-free detection of tumor cells in whole blood. ACS Sensors 2020, 5(2): 440-446.

[7] Ding LH, Chen X, He LL, Yu F, Yu SC, Wang J, Tian YM, Wang YL, Wu YJ*, Liu LE*, Qu LB*. Fluorometric immunoassay for the simultaneous determination of the tumor markers carcinoembryonic antigen and cytokeratin 19 fragment using two kinds of CdSe/ZnS quantum dot nanobeads and magnetic beads. Microchimica Acta 2020, 187(3): 171.

[8] Ding LH, Wu YJ, Liu W, Liu LE, Yu F, Yu SC, Tian YM, Feng JD, He LL*. Magnetic-assisted self-assembled aptamer/protein hybrid probes for efficient capture and rapid detection of cancer cells in whole blood. Talanta 2019, 205: 120129.


l 专利

[1] 丁丽华 (第发明人)一种自组装聚HRP探针、制备方法、试剂盒及其应用2020-02-07中国ZL 201710929595.2

[2] 丁丽华 (第发明人)血浆外泌体浓度和外泌体膜蛋白浓度的同步检测方法2023-07-21中国ZL 202111143245.6


l 学术奖励

[1] 丁丽华(2/3),医理工交叉学科博士研究生培养模式探索与实践。河南省教育科学研究优秀成果奖二等奖,2023。

[2] 第十“挑战杯”河南省大学生课外学术科技作品竞赛省级等奖,指导教师